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Methods & Protocols

If you would like to share your robust protocols with your colleagues, please email them to manager@stemcell.org.sg

Isolation of Tissue Stem Cells

  • Protocols Goodell Laboratory, Baylor College of Medicine, Houston
    (click here)
  • Protocols Thompson Laboratory, Wisconsin Institute for Discovery
    (click here)
  • Stem Cell Consortium Singapore Protocols
    (click here)
Gene Delivery into Stem Cells

  • Gene delivery to embryonic stem cells
    (Download)
  • Electroporation of Murine Embryonic Stem Cells: A Step-by-Step Guide (Dennie M. Tompers, Patricia A. Labosky Stem Cells 22: 243)
    (Download)
Human Embryonic Stem Cells

  • Methods for Expansion of Human Embryonic Stem Cells (Sun Kyung Oh et al., Stem Cells 23: 605)
    (Download)
  • Derivation and culture of hESC (Harvard lines, Cowan et al., N Engl J Med. 350:1353)
    (Download)

Additional protocols can be found on the NIH web site http://stemcells.nih.gov/research/protocols.asp and http://stemcells.nih.gov/research/nihresearch/scunit/protocols.htm.



 

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