Methods & Protocols
If you would like to share your robust protocols with your colleagues, please email them to manager@stemcell.org.sg Isolation of Tissue Stem Cells
- Protocols Goodell Laboratory, Baylor College of Medicine, Houston
(click here) - Protocols Thompson Laboratory, Wisconsin Institute for Discovery
(click here) - Stem Cell Consortium Singapore Protocols
(click here)
Gene Delivery into Stem Cells
- Gene delivery to embryonic stem cells
(Download) - Electroporation of Murine Embryonic Stem Cells: A Step-by-Step Guide (Dennie M. Tompers, Patricia A. Labosky Stem Cells 22: 243)
(Download)
Human Embryonic Stem Cells
- Methods for Expansion of Human Embryonic Stem Cells (Sun Kyung Oh et al., Stem Cells 23: 605)
(Download) - Derivation and culture of hESC (Harvard lines, Cowan et al., N Engl J Med. 350:1353)
(Download)
Additional protocols can be found on the NIH web site http://stemcells.nih.gov/research/protocols.asp and http://stemcells.nih.gov/research/nihresearch/scunit/protocols.htm.
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